Acta Univ. Agric. Silvic. Mendelianae Brun. 2017, 65, 205-210

https://doi.org/10.11118/actaun201765010205
Published online 2017-02-28

Detecting Pathogens of Verticillium Wilt in Winter Oilseed Rape Using ELISA and PCR – Comparison of the Two Methods and With Visual Stand Evaluation

Tomáš Spitzer, Pavel Matušinsky

Agrotest fyto, Ltd, Kromeriz, Czech Republic

During 2013–2015, oilseed rape samples from various locations in the Czech Republic were analysed for the presence of Verticillium wilt. Samples were evaluated in the lab using ELISA and PCR as well as by visual evaluation during the samples collection. A comparison of detection match in individual methods also was made. ELISA and PCR matched in detecting Verticillium wilt in 60 % of cases. For practical use, a higher match rate would be necessary in cases where samples were to be analysed in various laboratories using only one of these techniques. The possibility was demonstrated to use kits as well as primers not targeted specifically to just Verticillium longisporum but recording both main species on oilseed rape (V. longisporum and V. dahliae). The match rates of lab analyses with visual evaluation of stand infection were surprisingly high at 62 % for ELISA and 77 % for PCR. All three stand evaluation methods matched in 56 % of cases.

Funding

This publication was made possible by institutional support provided for the long‑term development of a research organization under Decision of the Ministry of Agriculture of the Czech Republic No. RO1116 from 28 February 2011 and was supported by the Ministry of Agriculture of the Czech Republic, Project No. QJ1310227.

References

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