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In 2003-2007 the issue of the forage production and quality on the permanent grassland was researched by the Crop Research Institute Prague, Research Station Jevíčko. The long-term small plot trials with tall oatgrass stand type (Arrhenatherion) were established in 2003 on a fluvisoil. Four types of utilisation were used in the trial: 1. Intensive (I₁) - 4 cuts per year (1^st cut on 15^th May, every next after 45 days); 2. Medium intensive (I₂) - 3 cuts per year (1^st cut on 30^th May, every next after 60 days); 3. Low intensive (I₃) - 2 cuts per year (1^st cut on 15^th June, 2^nd after 90 days); 4. Extensive (I₄) - 2 cuts per year (1^st cut on 30^th June, 2^nd after 90 days) and four levels of fertilizer application: F₀ = no fertilization; F_PK = P₃₀K₆₀N₀; F_PKN90 = P₃₀K₆₀+N₉₀; F_PKN180 = P₃₀K₆₀+N₁₈₀. The overall average DM production of grasslands over five years was 7.19 t.ha^-1. The lowest annual DM production is 6.77 t.ha^-1 during the intensive utilisation (I₁) and it increases towards the extensive (I₄) utilisation to 7.62 t.ha^-1, at all levels of fertilization. Application of phosphorus and potash fertilizers did not have significant effects on grassland DM yield. Application of N-fertilizer increased DM production (P_0.01) already on the level F_PKN90 to 8.49 t.ha^-1, on the level F_PKN180 DM matter still increased up to 9.51 t.ha^-1. Forage quality was highest during the intensive (I₁) utilisation and relatively lowest during the extensive (I₄) utilisation. The intensive (I₁) utilisation of permanent grassland improves OMD (P_0.01), concentration of CP and NEL and decreases fiber concentration (P_0.01) when compared with the extensive (I₄) utilisation. Fertilizing did not affect quality significantly except for CP concentration which increases with N-fertilization while NEL concentration decreases. Optimal utilisation intensity for dairy cows with annual production of 7-8 thousand kg of milk seems to be the intensive (I₁) utilisation with the level of fertilization F_PKN180 for given grass stand (plant society Arrhenatheretum). The forage from the medium intensively (I₂) utilized grassland meets quality parameters for suckler cows at the end of lactation period, the forage from less intensively and extensively (I₃, I₄) utilized grassland is suitable for cows only in the dry period in the form of hay.