Acta Univ. Agric. Silvic. Mendelianae Brun. 2020, 68(6), 929-936 | DOI: 10.11118/actaun202068060929

Molecular Evaluation of Three Populations of Farafra Sheep in Comparison to Ossimi and Rahmani Sheep Breeds

Talaat Bashandy1, Ahmed Hussein2, Mohamed Solma3, Ayman Kassab3, Hatem Hamdon3
1 Department of Genetics, Faculty of Agriculture, New Valley University, New Valley, Egypt
2 Department of Animal Production, Faculty of Agriculture, Assuit University, Asyut, Egypt
3 Department of Animal Production, Faculty of Agriculture, New Valley University, New Valley, Egypt

Molecular markers are the most ideal approach to study genetic diversity. Consequently, we utilized both ISSR and RAPD markers to assess genetic diversity and relationships among three different populations of Farafra, Ossimi and Rahmani Egyptian sheep breeds. Both ISSR and RAPD gave moderate polymorphism 41.3% and 48.51%, respectively. Besides, this value was consistent with the moderate value of the mean of polymorphism information content (0.16 and 0.20, respectively). Farafra-F and Farafra-D populations had the highest similarity which was 0.92 for ISSR and 0.90 for the RAPD marker. Furthermore, ISSR and RAPD constructed dendrogram separated all the studied sheep into two main clusters. All the three populations of Farafra breed combined into one main cluster, while the second cluster contained both Rahmani and Ossimi breeds. The used molecular markers were able to discriminate among evaluated sheep and displayed that Farafra breed more closely related to Ossimi than Rahmani breed.

Keywords: dendrogram, Farafra sheep, genetic diversity, ISSR, molecular markers, RAPD
Grants and funding:

We are grateful to the Faculty of Agriculture, New Valley University, Egypt for the financial support of this work.

Received: August 20, 2020; Accepted: November 18, 2020; Published: December 17, 2020  Show citation

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Bashandy, T., Hussein, A., Solma, M., Kassab, A., & Hamdon, H. (2020). Molecular Evaluation of Three Populations of Farafra Sheep in Comparison to Ossimi and Rahmani Sheep Breeds. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 68(6), 929-936. doi: 10.11118/actaun202068060929
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