Acta Univ. Agric. Silvic. Mendelianae Brun. 2020, 68(5), 851-857 | DOI: 10.11118/actaun202068050851

The Effects of Potassium Silicate as a Component of Nutrient Medium for Selected in Vitro Cultures of Prunus and Corylus Genera

Eliška Kadlecová1, Miroslav Baránek1, Samuel Magnús2, Filip Gazdík1
1 Mendeleum - Department of Genetics, Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech Republic
2 Department of Fruit Growing, Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech Republic

Even though silicon is frequent compound in soil, its use in plant nutrient media is rare. Based on known physiological role and up to now performed studies it seems that silicon has a good potential to improve growth characteristics of in vitro cultivated plants. Before practical application, however, it is always necessary to assess the optimal conditions of application with regard to the fact that plant reactions to different chemicals added to nutritional media can vary on the species or even cultivar level. The presented study evaluate effects of potassium silicate used in in vitro cultivation media on growth parameters of Prunus persica × Prunus davidiana 'Cadaman,' Prunus × amygdalopersica 'GF 677' and Corylus avellana 'Tonda di Giffoni' genotypes, which are frequently subject of commercial in vitro multiplication.
In fact, four different concentrations of potassium silicate was added to the multiplication media, control medium was left silicon-free. Three different characteristics were observed during cultivation - number of new shoots per explant, weight of a new plant and length of new shoots, from which number of new shoots per explant was considered the most important factor.
In all cases a positive effects of potassium silicate on the condition and other growth parameters of treated cultures were observed. In 'Cadaman' culture significant growth changes appeared on media with 20 mg.l-1 potassium silicate, which can be recommended for future applications. For 'GF 677' the best results were obtained on media with 2 mg.l-1 which can be recommended for improving condition and number of shoots on new plants. For hazelnut genotype 'Tonda di Giffoni' best results were obtained on media with 10 mg.l-1 of potassium silicate, but also use of 5 mg.l-1 of potassium silicate significantly improved growth parameters. Generally, presented study provides important and practically useful insights into the practical use of silicon in cultivation media designated for commercial in vitro micropropagation.

Keywords: silicon, micropropagation, tissue culture, in vitro, Prunus, Corylus, media
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This work was supported by IGA - ZF/2018 - DP004 of Mendel University in Brno, Czech Republic. This work was partially supported by using the infrastructure obtained from the project CZ.02.1.01/0.0/0.0/16_017/0002334 Research Infrastructure for Young Scientists is co-financed from Operational Programme Research, Development and Education.

Received: March 18, 2020; Accepted: September 16, 2020; Published: November 1, 2020  Show citation

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Kadlecová, E., Baránek, M., Magnús, S., & Gazdík, F. (2020). The Effects of Potassium Silicate as a Component of Nutrient Medium for Selected in Vitro Cultures of Prunus and Corylus Genera. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 68(5), 851-857. doi: 10.11118/actaun202068050851
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