Acta Univ. Agric. Silvic. Mendelianae Brun. 2020, 68(2), 305-309 | DOI: 10.11118/actaun202068020305

A New Viewpoint on Genetic Diversity in Prestice Black-Pied Pig: Did the Breed Suffer from a Bottleneck?

Lenka Falková1, Irena Vrtková1, Štěpán Vrtek
1 Laboratory of Agrogenomics, Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic

The research was aimed at determination of genetic variability of Prestice Black-Pied (PC) pig breed (Czech national breed and genetic resource) and to evaluate possible presence of recent bottleneck in this closed small pig population. One hundred and eighty of breeding boars were analysed by eleven tetramer Short Tandem Repeats (STR) panel specifically developed for the genotyping of breeding livestock. Despite the fact that appearance of rare alleles, which may be relatively increased after recent bottleneck, was discovered, the heterozygosity excess was not significant. The PC breed has not undergone recent bottleneck and remained at mutation-drift equilibrium.

Keywords: bottleneck effect, genetic variability, genetic resource, Prestice Black-Pied pig
Grants and funding:

This paper was supported by NAZV QK1910400.

Received: February 6, 2020; Accepted: March 31, 2020; Published: April 29, 2020  Show citation

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Falková, L., Vrtková, I., & Vrtek, Š. (2020). A New Viewpoint on Genetic Diversity in Prestice Black-Pied Pig: Did the Breed Suffer from a Bottleneck? Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis68(2), 305-309. doi: 10.11118/actaun202068020305
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References

  1. CORNUET, J. M. and LUIKART, G. 1996. Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data. Genetics, 144(4): 2001-2014. Go to original source...
  2. FALKOVÁ, L. and VRTKOVÁ, I. 2017. Searching for breed specific genetic markers of national genetic resource - the Přeštice Black-Pied Pig. In: Research in Pig Breeding. 16th International workshop. 24-25 October 2019, Kostelec nad Orlicí - Vrbice.
  3. FERREIRA, E., SOUTO, L., SOARES, A. M. V. M. and FONSECA, C. 2009. Genetic structure of the wild boar population in Portugal: Evidence of a recent bottleneck. Mammalian Biology, 74(4): 274-285. DOI: 10.1016/j.mambio.2008.05.009 Go to original source...
  4. FRANTZ, L. A. F., SCHRAIBER, J. G., MADSEN, O., MEGENS, H.-J., CAGAN, A., BOSSE, M., PAUDEL, Y., CROOIJMANS, R. P. M. A., LARSON, G. and GROENEN, M. A. M. 2015. Evidence of long-term gene flow and selection during domestication from analyses of Eurasian wild and domestic pig genomes. Nature Genetics, 47(10): 1141-1148. DOI: 10.1038/ng.3394 Go to original source...
  5. GHOLIZADEH, M., RAHIMI, G., MAINJI, G. R. and ZADEH, H. S. 2008. Potential use of molecular markers in the genetic improvement of livestock. Asian Journal of Animal and Veterinary Advances, 3: 120-128. DOI: 10.3923/ajava.2008.120.128 Go to original source...
  6. HARCET, M., DIKIC, M. and GAMULIN, V. 2006. Low genetic diversity of the Turopolje pig breed. Food Technology and Biotechnology, 44(1): 105-109.
  7. KRAMARENKO, A. S., GLADYR, E. A., KRAMARENKO, S. S., PIDPALA, T. V., STRIKHA, L. A. and ZINOVIEVA, N. A. 2018. Genetic diversity and bottleneck analysis of the Red Steppe cattle based on microsatellite markers. Ukrainian Journal of Ecology, 8(2): 12-17.
  8. KRUPA, E., KRUPOVÁ, Z., ŽÁKOVÁ, E., KASARDA, R. and SVITÁKOVÁ, A. 2015. Population analysis of the local endangered Přeštice Black-Pied pig breed. Poljoprivreda/Agriculture, 21(supplement): 155-158. DOI: 10.18047/poljo.21.1.sup.36 Go to original source...
  9. LAVAL, G., IANNUCCELLI, N., LEGAULT, C., MILAN, D., GROENEN, M. A. M., GIUFFRA, E., ANDERSSON, L., NISSEN, P. H., JARGENSEN, C. B., BEECKMANN, P., GELDERMANN, H., FOULLEY, J. L., CHEVALET, C. and OLLIVIER, L. 2000. Genetic diversity of eleven European pig breeds. Genetics Selection Evolution, 32: 187-203. DOI: 10.1186/1297-9686-32-2-187 Go to original source...
  10. MARUYAMA, T. and FUERST, P. A. 1985. Population bottlenecks and nonequilibrium models in population genetics. II. number of alleles in a small population that was formed by a recent bottleneck. Genetics, 111(3): 675-689. Go to original source...
  11. PIRY, S., LUIKART, G. and CORNUET, J. M. 1999. BOTTLENECK: A computer program for detecting recent reductions in the effective population size using allele frequency data. Journal of Heredity, 90(4): 502-503. DOI: 10.1093/jhered/90.4.502 Go to original source...
  12. TORO, M. A., GARCIA-CORTES, L. A. and LEGARRA, A. 2011. A note on the rationale for estimating genealogical coancestry from molecular markers. Genetics Selection Evolution, 43: 27. DOI: 10.1186/1297-9686-43-27 Go to original source...
  13. VÁCLAVKOVÁ, E., ROZKOT, M. and DOSTÁLOVÁ, A. 2012. Přeštické černostrakaté prase. Živé dědictví po předcích. Praha Uhříněves: VÚŽV v.v.i.
  14. VRTKOVÁ, I. 2015. Genetic admixture analysis in Prestice Black-Pied pigs. Archives Animal Breeding, 58: 115-121. DOI: 10.5194/aab-58-115-2015 Go to original source...
  15. VRTKOVÁ, I, STEHLÍK, L., PUTNOVÁ, L., KRATOCHVÍLOVÁ, L. and FALKOVÁ, L. 2012. Genetic structure in three breeds of pigs populations using microsatellite markers in the Czech Republic. Research in Pig Breeding, 6(2): 83-87.
  16. VRTKOVÁ, I., FILISTOWICZ, A., BUCZYŃSKI, J. T., VRTEK, Š. and FALKOVÁ, L. 2017. Parameters of variability and diversity of tetrameric STRs for practical use at Zlotnicka White and Zlotnicka Spotted pigs. Animal Science Papers and Reports, 35: 83-87.
  17. WALDICK, R. C., KRAUS, S., BROWN, M. and WHITE, B. N. 2002. Evaluating the effects of historic bottleneck events: an assessment of microsatellite variability in the endangered, North Atlantic right whale. Molecular Ecology, 11: 2241-2249. DOI: 10.1046/j.1365-294X.2002.01605.x Go to original source...
  18. WHITEHOUSE, A. M. and HARLEY, E. H. 2001. Post-bottleneck genetic diversity of elephant populations in South Africa, revealed using microsatellite analysis. Molecular Ecology, 10(9): 2139-2149. DOI: 10.1046/j.0962-1083.2001.01356.x Go to original source...

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