Acta Univ. Agric. Silvic. Mendelianae Brun. 2019, 67(6), 1453-1456 | DOI: 10.11118/actaun201967061453

Micropropagation of Incarvillea delavayi Bureau et Franchet (Bignoniaceae)

Tomáš Hovorka¹, Iva Viehmannová¹, Jan Vitamvas², Petra Hlásná Čepková³, Eloy Fernández¹

¹ Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamýcká 129, 165 21 Prague, Czech Republic

² Department of Forest Ecology, Faculty of Forestry and Wood Sciences, Czech University of Life Sciences Prague, Kamýcká 129, 165 21 Prague, Czech Republic

³ Gene Bank, Crop Research Institute, Drnovská 507/73, 161 06 Prague, Czech Republic

A simple and efficient protocol for micropropagation of Incarvillea delavayi (Bignoniaceae), an ornamental plant from China, was developed in this study. As initial plant material, adventitious shoots were used. Effect of 0, 0.1, 0.5 and 1 mg l^-1 BAP in half-strength MS medium on shoot multiplication was studied. For rooting, 0.1 and 0.3 mg l^-1 NAA were tested. The highest shoot number (8.60 ± 0.78 shoots per explant) and plant height (5.50 ± 0.31 cm) were obtained on medium supplemented with 1 mg l^-1 BAP. The shoots optimally rooted on medium with addition of 0.3 mg l^-1 NAA (8.48 ± 0.51 roots per shoot; root length 1.64 ± 0.18 cm). Well rooted plants were transferred ex vitro. Eight weeks after ex vitro transfer, 62.5% plants survived. The protocol optimized here, can be used for large-scale propagation of ornamental plant I. delavayi.

Keywords: α-naphthalenacetic acid, 6-benzylaminopurine, Bignoniaceae, Incarvillea delavayi, micropropagation, ornamental plant

Grants and funding:

The study was supported by the Internal Grant Agency of the Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague IGA (Project No. 20195002).

Received: June 10, 2019; Accepted: October 25, 2019; Published: December 22, 2019  Show citation