Acta Univ. Agric. Silvic. Mendelianae Brun. 2019, 67, 179-187

https://doi.org/10.11118/actaun201967010179
Published online 2019-02-28

Probiotic Candidates Yeast Isolated from Dangke – Indonesian Traditional Fermented Buffalo Milk

Gemilang Lara Utama1, Siska Meliana1, Mohamad Djali1, Tri Yuliana1, Roostita L. Balia2

1Faculty of Agro‑Industrial Technology, Universitas Padjadjaran, Bandung, 45363, Indonesia
2Faculty of Animal Husbandry, Universitas Padjadjaran, Bandung, 45363, Indonesia

Received August 17, 2018
Accepted November 22, 2018

The aim of the study was to isolate and identify the yeast isolated from Dangke and its potential as probiotics. The purified isolates obtained were identified based on observations of macroscopic characteristics of colonies and microscopic cells. The ability as a probiotic yeast is obtained by testing the resistance towards acid conditions, bile salt resistance test and aggregation ability test against pathogenic bacteria using Salmonella sp. The yeast isolates were identified using the RapID Yeast Plus System. The isolation result was obtained D.10.3.d isolate that identified as Candida guilliermondii which showed probiotic characteristic. The yeast colony is round, cream‑colored, smooth surfaces, low convex elevations and entire edges, capable of growing on mediums with the pH of 4, containing 1 % and 5 % of bile salts and having the ability to aggregate Salmonella sp. at 15, 60, and 180 minutes.

Funding

This research/article’s publication is supported by the United States Agency for International Development (USAID) through the Sustainable Higher Education Research Alliance (SHERA) Program for Universitas Indonesia’s Scientific Modeling, Application, Research and Training for City-centered Innovation and Technology (SMART CITY) Project, Grant #AID-497-A1600004, Sub Grant #IIE-00000078-UI-1.

References

43 live references