RT Journal Article SR Electronic A1 Doležal, Petr A1 Dvořáček, Jan A1 Dvořáčková, Jitka A1 Poštulka, Roman A1 Doležal, Jan A1 Szwedziak, Katarzyna T1 The use of yeast culture in feeding of dairy cows JF Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis YR 2014 VO 58 IS 5 SP 75 OP 82 DO 10.11118/actaun201058050075 UL https://acta.mendelu.cz/artkey/acu-201005-0009.php AB The aim of this study was to determine the effect of a yeast culture (Saccharomyces cerevisiae CNCM I - 1077) addition in the diet of dairy cows on their rumen fermentation. Animals received a diet consisting of good maize silage with a higher dry matter content (18 kg), 6 kg of lucerne haylage, 5 kg of pea - silage, 3 kg of ensiled high moisture crimped corn, 1 kg of meadow hay, rape-cake 1 kg, brewer's grain silage 4 kg, and 8.0 kg feed mixture. The yeast culture was added to the mixture in the dose 2.8*10<sup>10</sup> cfu/day and cow. The supplement of yeast culture showed a positive effect on ruminal VFA production by experimental group cows in comparison with control, higher production (P &lt; 0.01) of acetic acid (58.50 ± 1.2583<sup>b</sup> vs. 51.833 ± 2.409<sup>a</sup> %), and lower production of propionic and butyric acid (24.667 ± 1.3744<sup>a</sup> vs. 28.833 ± 1.863<sup>b</sup> %). The average utilisation of ammonia was higher by cows in treated group (12.267 ± 0.754 mmol/L), but difference was not significant. The difference in number of protozoa of cows in the control and experimental groups was significant (P &lt; 0.05) different (308.333 ± 24.390<sup>a</sup> vs. 342.333 ± 10.9341<sup>b</sup> ths /1 ml of rumen fluid).