RT Journal Article
SR Electronic
A1 Stavělíková, Helena
A1 Hanáček, Pavel
A1 Vyhnánek, Tomáš
T1 The morphological description and DNA tools analysis: for detection of duplications in the Czech germplasm collection of pepper (Capsicum annuum L.)
JF Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
YR 2014
VO 58
IS 1
SP 191
OP 198
DO 10.11118/actaun201058010191
UL https://acta.mendelu.cz/artkey/acu-201001-0023.php
AB The pepper (Capsicum annuum L.) is very popular annual vegetable either for fresh consume either as spice after drying and grinding. The fruit contains high amounts of vitamin C, provitamin A, E, P (citrin), B<sub>1</sub> (thiamine), B<sub>2</sub> (riboflavin) and B<sub>3</sub> (niacin). Crop Research Institute (CRI), Department of Vegetable and Special Crops, Olomouc, the Czech Republic is the holder of the collection of pepper genetic resources. The collection of pepper consists of 504 accessions, currently. It is necessary to find duplications within collection for effective work with genetics resources. For analyses totally 41 accessions were chosen. These were divided into ten groups according name: 1. Astrachanskij, 2. Aufrechte Cayenne, 3. Bogyisloi, 4. Hatvani, 5. Japan Hontakka, 6. Japan Madarszen, 7. Kalocsai Fuszer (Edes), 8. Konservnyj Belyj 289, 9. Tetenyi and 10. Vinedale. Two approaches were used for the detection of duplications - morphological description and polymorphism of DNA. The accessions were characterized for 54 morphological traits: 1 character in seedlings, 8 characters in the plants, 10 characters in leaves, 10 characters in flowers and 25 characters in fruits. The polymorphism of DNA was analysed using the SSR (Simple Sequence Repeats) method with 8 SSR markers (Hpms 1-1, Hpms 1-5, Hpms 1-168, Hpms 1-172, Hpms 1-274, Hpms 2-21, Cams 163 and Cams 647) which are localised on different chromosomes. The results from DNA analysis were complemented with the morphological characterization. Possible duplications were in 4 groups: 1. Astrachanskij, 4. Hatvani, 5. Japan Hontakka and 7. Kalocsai Fuszer (Edes). This work is the first step for the determination of duplications in the Czech germplasm collection of pepper.