PT - JOURNAL ARTICLE AU - Pospíšilová, Ľubica AU - Fasurová, Naděžda AU - Liptaj, Tibor AU - Jurica, Lubomír TI - Humic acids characterization by EDXS and <sup>13</sup>C NMR spectroscopy DP - 2014 Oct 5 TA - Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis PG - 139--146 VI - 58 IP - 1 AID - 10.11118/actaun201058010139 IS - 12118516 AB - Determination of humic acids spectral characteristics performed fast and convenient method for their quality evaluation. Humic acids (HA) were isolated from five different soil types (Luvi-haplic Chernozem, Haplic Luvisol, Haplic Cambisol, Leptic Cambisol and Eutric Cambisol) and from compost. Basic soil characteristics were determined by commonly used methods. Fractional compositon of humus showed that humic acids content was decreasing in order: compost &gt; Luvi-haplic Chernozem &gt; Haplic Luvisol &gt; Eutric Cambisol &gt; Haplic Cambisol &gt; Leptic Cambisol. Isolated humic acids preparations were characterized comparatively by chemical and spectroscopic methods. Elemental analysis showed the highest carbon content in Luvi-haplic Chernozem HA. The lowest carbon amount was in Eutric Cambisol. Energy-dispersive X-ray spectroscopy detected elements in situ in HA molecule. All samples contained Fe, Cu, Zn, Ti, Ca, K, S, P, Si and Br. Practically no differences in elements content between soil humic substances and compost were detected. The amount of aromatic carbon was higher in Luvi-haplic Chernozem to compare with other samples. Humic acids isolated from Cambisols, on the basis <sup>13</sup>C NMR spectroscopic data, showed that all samples contained less aromatic carbon and significantly more aliphatic carbon.