RT Journal Article
SR Electronic
A1 Sláma, Petr
T1 Application of Western Blot for detection of neutrophil apoptosis-related proteins
JF Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
YR 2014
VO 57
IS 1
SP 97
OP 104
DO 10.11118/actaun200957010097
UL https://acta.mendelu.cz/artkey/acu-200901-0010.php
AB The aim of this study was to evaluate suitability of using Western Blot for detection of neutrophil apoptosis and neutrophil apoptosis-related proteins, respectively. Neutrophils were isolated from blood of healthy adult donors and incubated with G-CSF (granulocyte colony stimulating factor), GM-CSF (granulocyte-macrophage colony stimulating factor), ATP (adenosine triphosphate) and FMLP (N-formyl-methionyl-leucyl-phenylalanine). The neutrophils were incubated 4, 8 and 20 hours at 37 °C. In this assay, an expression of Mcl-1 (myeloid cell leukemia 1), XIAP (X-linked inhibitor of apoptosis) and gelsolin was analysed by Western Blot method. The results showed that Western Blot is a suitable method for detection of neutrophil apoptosis-related proteins and detection of neutrophil apoptosis, respectively.