PT  - JOURNAL ARTICLE
AU  - Sláma, Petr
TI  - Application of Western Blot for detection of neutrophil apoptosis-related proteins
DP  - 2014 Oct 14
TA  - Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
PG  - 97--104
VI  - 57
IP  - 1
AID - 10.11118/actaun200957010097
IS  - 12118516
AB  - The aim of this study was to evaluate suitability of using Western Blot for detection of neutrophil apoptosis and neutrophil apoptosis-related proteins, respectively. Neutrophils were isolated from blood of healthy adult donors and incubated with G-CSF (granulocyte colony stimulating factor), GM-CSF (granulocyte-macrophage colony stimulating factor), ATP (adenosine triphosphate) and FMLP (N-formyl-methionyl-leucyl-phenylalanine). The neutrophils were incubated 4, 8 and 20 hours at 37 °C. In this assay, an expression of Mcl-1 (myeloid cell leukemia 1), XIAP (X-linked inhibitor of apoptosis) and gelsolin was analysed by Western Blot method. The results showed that Western Blot is a suitable method for detection of neutrophil apoptosis-related proteins and detection of neutrophil apoptosis, respectively.