RT Journal Article SR Electronic A1 Szewczuk, Małgorzata A1 Zych, Sławomir A1 Chaberski, Ryszard T1 Effect of growth hormone-releasing hormone gene polymorphism (GHRH/HaeIII) on milk performance in polish holstein-friesian cows JF Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis YR 2014 VO 56 IS 4 SP 177 OP 182 DO 10.11118/actaun200856040177 UL https://acta.mendelu.cz/artkey/acu-200804-0020.php AB The objective of this study was to evaluate the relationship between the polymorphism within GHRH gene (GHRH/HaeIII) and milk production traits for Polish Holstein-Friesian using PCR-RFLP technique for genotyping. The molecular background of this mutation was defined as the A→C transversion at the intron 2. A total of 220 cows were examined. The following frequencies were established: 0.0227 for genotype AA, 0.3227 for AB, and 0.6546 for BB; 0.1841 for the allele GHRH<sup>A</sup> and 0.8159 for GHRH<sup>B</sup>. A highly significant association was found between the GHRH/HaeIII polymorphism and milk performance. The BB-genotype cows were characterised by a higher fat yield and percentage (P ≤ 0.01).