PT  - JOURNAL ARTICLE
AU  - Szewczuk, Małgorzata
AU  - Zych, Sławomir
AU  - Chaberski, Ryszard
TI  - Effect of growth hormone-releasing hormone gene polymorphism (GHRH/HaeIII) on milk performance in polish holstein-friesian cows
DP  - 2014 Nov 8
TA  - Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
PG  - 177--182
VI  - 56
IP  - 4
AID - 10.11118/actaun200856040177
IS  - 12118516
AB  - The objective of this study was to evaluate the relationship between the polymorphism within GHRH gene (GHRH/HaeIII) and milk production traits for Polish Holstein-Friesian using PCR-RFLP technique for genotyping. The molecular background of this mutation was defined as the A→C transversion at the intron 2. A total of 220 cows were examined. The following frequencies were established: 0.0227 for genotype AA, 0.3227 for AB, and 0.6546 for BB; 0.1841 for the allele GHRH<sup>A</sup> and 0.8159 for GHRH<sup>B</sup>. A highly significant association was found between the GHRH/HaeIII polymorphism and milk performance. The BB-genotype cows were characterised by a higher fat yield and percentage (P ≤ 0.01).