PT - JOURNAL ARTICLE AU - Burdychová, Radka AU - Hoferková, Petra TI - Monitoring of psychrotrophic microorganisms in raw milk DP - 2014 Nov 8 TA - Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis PG - 21--28 VI - 56 IP - 4 AID - 10.11118/actaun200856040021 IS - 12118516 AB - The group of psychrotrophic microorganisms belongs to the microorganisms representing a risk for human health as well as a risk of milk and milk products spoilage. Some genus are considered to be significant producers of proteolytic and lipolytic enzymes. In this work, we analysed raw milk samples (n = 109) originated from 26 different suppliers from the area of North and Middle Moravia. The screening was performed from March 2007 to February 2008. The total bacterial counts (TBC) ranged between 3.2 × 10<sup>3</sup> to 8.3 × 10<sup>6</sup> CFU/ml. The psychrotrophic bacterial counts (PBC) ranged between 1.0 × 10<sup>3</sup> to 8.2 × 10<sup>6</sup> CFU/ml. Total of 48.62 % and 48.62 % of samples exceeded the hygienic limit in raw milk for TBC and PBC, respectively. The correlation between TBC and PBC was highly significant (r = 0.87).Significantly higher (P &lt; 0.05) numbers of psychrotrophic microorganisms were detected in summer months. The identification of isolates was carried out and all strains were sreened for ability to produce proteolytic and lipolytic enzymes. The most commonly identified genus in raw milk was of the genus Pseudomonas. The ability to produce proteases or lipases was found at 76 % identified bacterial strains.