RT Journal Article
SR Electronic
A1 Sláma, Petr
A1 Sládek, Zbyšek
A1 Ryšánek, Dušan
A1 Burešová, Ivana
T1 The effect of temperature on apoptosis of bovine blood eosinophil granulocytes in vitro
JF Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
YR 2014
VO 56
IS 1
SP 173
OP 178
DO 10.11118/actaun200856010173
UL https://acta.mendelu.cz/artkey/acu-200801-0021.php
AB The aim of the study was to evaluate the effect of temperature on apoptosis of bovine blood eosinophil granulocytes in vitro. Heparinised bovine blood was incubated for 1, 4 and 24 h under following temperatures: 4, 23 and 37 °C. UV irradiation was used as positive control of apoptosis. Eosinophil granulocytes apoptosis was detected by flow cytometry after simultaneous staining with Annexin-V and propidium iodide. From selected temperatures, 4 °C induced the eosinophil granulocytes apoptosis least. The proportion of apoptotic eosinophil granulocytes amounted to (mean ± SD) 1.65 ± 0.46%; 1.76 ± 0.36%; 4.78 ± 1.70% after 1, 4 and 24 h incubation, respectively.