PT  - JOURNAL ARTICLE
AU  - Sláma, Petr
AU  - Sládek, Zbyšek
AU  - Ryšánek, Dušan
AU  - Burešová, Ivana
TI  - The effect of temperature on apoptosis of bovine blood eosinophil granulocytes in vitro
DP  - 2014 Nov 17
TA  - Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
PG  - 173--178
VI  - 56
IP  - 1
AID - 10.11118/actaun200856010173
IS  - 12118516
AB  - The aim of the study was to evaluate the effect of temperature on apoptosis of bovine blood eosinophil granulocytes in vitro. Heparinised bovine blood was incubated for 1, 4 and 24 h under following temperatures: 4, 23 and 37 °C. UV irradiation was used as positive control of apoptosis. Eosinophil granulocytes apoptosis was detected by flow cytometry after simultaneous staining with Annexin-V and propidium iodide. From selected temperatures, 4 °C induced the eosinophil granulocytes apoptosis least. The proportion of apoptotic eosinophil granulocytes amounted to (mean ± SD) 1.65 ± 0.46%; 1.76 ± 0.36%; 4.78 ± 1.70% after 1, 4 and 24 h incubation, respectively.