RT Journal Article SR Electronic A1 Doležal, Jan A1 Doležal, Petr T1 Digestibility of organic matter of total mixed rations with the supplementation of yeast culture using the in vitro method JF Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis YR 2014 VO 55 IS 5 SP 59 OP 64 DO 10.11118/actaun200755050059 UL https://acta.mendelu.cz/artkey/acu-200705-0008.php AB We conducted this study to evaluate the new in vitro system, DAISY<sup>II</sup> to determine organic matter (OM) digestibility in ruminant feedstuffs with and without addition of the yeast culture (RUMEX) in the amount of 0 grams; 0.30 grams and 2.40 grams/2L of rumen fluid. Three types of total mix rations (TMR) were used in the experiment. Feedstufs samples were mixed in the ruminal fluid of cows. The material was incubated at 39 degrees of Celsius for 48 hours. Results from the DAISY<sup>II</sup> were compared with one another. Higher fibrous material content in 1 kilogram of dry matter (145.4 grams or 218.6 grams) and less energy ME (10.24 MJ or 11.88 MJ/kilogram of dry matter) in each TMR gave rise to worse organic matter digestibility. The highest digestibility of organic matter was noted at TMR 1 (81.04 % (0 g) or 83.47 % (0.30 g) or 84.30 % (2.4 g) with the addition of the yeast culture) and the lowest digestibility was noted at TMR 3 (78.98 % (0 g) or 82.49 % (0.30 g) or 82.95 % (2.4 g)). The supplement of the yeast culture in the amount of 0.3 grams (P