PT  - JOURNAL ARTICLE
AU  - Bezdíček, Jiří
TI  - Allele and genotype frequencies of milkprotein kappa-casein (CSN3) in artifical insemination bulls of Czech Fleckvieh and Holstein breed
DP  - 2014 Nov 26
TA  - Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
PG  - 17--22
VI  - 55
IP  - 5
AID - 10.11118/actaun200755050017
IS  - 12118516
AB  - This thesis was meant to evaluate allele and genotype frequencies of kappa-casein (CSN3) in artificial insemination bulls in the Czech Republic of Czech Fleckvieh breed (n = 133) and Holstein breed (n = 58). Studied bulls were born between 1990 and 2005. Calculation was made by PowerMarker (Liu K., Muse S.V. 2005). Genotype frequencies established in Czech Fleckvieh bulls were AA = 0.391 AB = 0.4887 AE = 0.0075 BB = 0.0902 BE = 0.0226 and EE = 0 and allele frequencies were A = 0.6391 B = 0.3459 E = 0.015. Genotype frequencies established in Holstein bulls were AA = 0.5517 AB = 0.2759 AE = 0.1207 BB = 0 BE = 0.0345 and EE = 0.0172 and allele frequencies A = 0.75 B = 0.1552 and E = 0.0948. In Holstein breed we found higher frequency of E allele and higher frequency of genotypes with this allele. Genetic diversity, heterozygosis and PIC values in Czech Fleckvieh Bulls were following 0.4717; 0.5188 and 0.3737 and 0.4044; 0.4310 and 0.3668 in Holstein Bulls. These results show the higher genetic variability in bulls of Czech Fleckvieh breed. Results of χ<sup>2</sup> test (Czech Fleckvieh 4.58; Holstein 2.55) show genetical disequilibrium in the Bulls both examined breeds used in artificial insemination in the Czech Republic.