PT  - JOURNAL ARTICLE
AU  - Burdychová, Radka
TI  - Identification and typization of bacteria of the genus Enterococcus supposed to be used for the production of functional foods
DP  - 2014 Nov 27
TA  - Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
PG  - 9--14
VI  - 55
IP  - 2
AID - 10.11118/actaun200755020009
IS  - 12118516
AB  - In this study, the species identification of 12 probiotic strains of the genus Enterococcus from Culture Collection of Dairy Microorganisms Lactoflora (CCDM, Milcom, Tábor, Czech Republic) were done using PCR described by DUTKA-MALEN et al. (1995). All strains were classified to be of the genus Enterococcus and species E. faecium. These strains are supposed to be used as probiotics for the production of functional foods. According to the fact that E. faecium was described to have decarboxylase activity responsible for biogenic amine production in fermented products, the presence of genes coding for microbial tyrosine and histidine decarboxylase was screened in all strains using PCR described by COTON et al. (2004). Whereas the presence of DNA sequences for histidine decarboxylase was not detected in any strain, specific DNA sequences coding for tyrosine decarboxylases were detected in all tested strains. When applying as starter probiotic cultures to fermented milk products, the production of biogenic amine tyramine have to be observed during both fermentation and storage.