RT Journal Article
SR Electronic
A1 Burdychová, Radka
T1 Screening of selected starter cultures for the presence of DNA sequences coding for tyrosine decarboxylase
JF Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
YR 2014
VO 54
IS 5
SP 7
OP 12
DO 10.11118/actaun200654050007
UL https://acta.mendelu.cz/artkey/acu-200605-0001.php
AB Here, seven different starter cultures used in the production of fermented sausages were screened for the presence or absence of specific DNA sequences coding for tyrosine decarboxylase. PCR with the a set of specific primers TDC2/TDC5 (COTON et al., 2004) was used. The PCR analysis of DNA from two starter cultures confirmed the presence of DNA sequences for tyrosine decarboxylase. A detailed analysis of the starter cultures showed that DNA sequences for tyrosine decarboxylase are contained in genomic DNA of Lactobacillus curvatus and Lactobacillus sakei.These results show suitability of the described PCR method for the screening of starter cultures for the presence of the gene for tyrosine decarboxylase that is responsible for the production of the biogenic amine tyramine.