PT  - JOURNAL ARTICLE
AU  - Burdychová, Radka
TI  - Screening of selected starter cultures for the presence of DNA sequences coding for tyrosine decarboxylase
DP  - 2014 Dec 3
TA  - Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
PG  - 7--12
VI  - 54
IP  - 5
AID - 10.11118/actaun200654050007
IS  - 12118516
AB  - Here, seven different starter cultures used in the production of fermented sausages were screened for the presence or absence of specific DNA sequences coding for tyrosine decarboxylase. PCR with the a set of specific primers TDC2/TDC5 (COTON et al., 2004) was used. The PCR analysis of DNA from two starter cultures confirmed the presence of DNA sequences for tyrosine decarboxylase. A detailed analysis of the starter cultures showed that DNA sequences for tyrosine decarboxylase are contained in genomic DNA of Lactobacillus curvatus and Lactobacillus sakei.These results show suitability of the described PCR method for the screening of starter cultures for the presence of the gene for tyrosine decarboxylase that is responsible for the production of the biogenic amine tyramine.