PT  - JOURNAL ARTICLE
AU  - Vlašínová, Helena
AU  - Havel, Ladislav
AU  - Klemš, Marek
AU  - Procházka, Stanislav
TI  - Uptake and metabolism of 2,4-dichlorophenoxyacetic acid during induction of Norway spruce (Picea abies (L.) Karst.) somatic embryogenesis
DP  - 2014 Dec 22
TA  - Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
PG  - 169--174
VI  - 53
IP  - 5
AID - 10.11118/actaun200553050169
IS  - 12118516
AB  - Uptake and metabolism of 2,4-dichlorophenoxyacetic acid (2,4-D) into zygotic embryos of Norway spruce (Picea abies (L.) Karst.) were studied on medium used for induction of somatic embryogenesis. The main task of this work was to study effect of longitudinal bisection of embryos, which was found as increasing the subsequent induction of embryogenic cultures. The maximal uptake of 2,4-D per one embryo was detected after 16 hours on medium in bisected embryos. The bisection increased 2,4-D uptake per embryo in first 16<sup>th</sup> hours, but then increased its release back to the medium. The metabolism of 2,4-D in bisected embryos was lower than in intact ones during first two days of culture.