Acta Univ. Agric. Silvic. Mendelianae Brun. 2020, 68, 679-686
Published online 2020-08-30

Micropropagation of Camptotheca Acuminata Decne (Nyssaceae) – Endangered Ornamental and Medicinal Tree

Lilyana Nacheva1, Nataliya Dimitrova1, Valeria Ivanova2, Fuliang Cao3, Zunling Zhu3

1Laboratory of Тissue Cultures, Institute of Fruitgrowing of Plovdiv, 4004 Plovdiv, 12 “Ostromila” str., Bulgaria
2Department of Horticulture, Faculty of Horticulture with Viticulture, Agricultural University, 12 Mendeleev str., 4000 Plovdiv, Bulgaria
3Nanjing Forestry University, 159 Lonpan Road, Nanjing, 210037, China

Received August 11, 2019
Accepted July 2, 2020

Camptotheca acuminata Decne (Nyssaceae) (happy tree, tree of life, cancer tree) is a rapidly growing deciduous and endangered tree endemic to east Tibet and southern China (Liu et al., 2002). C. acuminata is an ornamental tree with monopodial growth, beautiful leaves and shade- and cold- tolerant in their natural environment. Recently, its demand has grown rapidly due to secondary metabolites production like Camptothecin and its derivatives with anti cancer and antivirus activity. The aim of the present study was to develop an efficient protocol for in vitro micropropagation of this valuable plant. Different cultural media based on both MS (Murashige and Skoog, 1962) or DKW (Driver and Kuniyuki, 1984) formulations with different cytokinins (6-benzylaminopurine, BAP or 2-isopentenyladenine, 2iP or meta-topolin, mT) have been involved. The aromatic cytokinin meta-topolin stimulated plant growth. The optimum multiplication rate of Camptotheca shoots occurred on the DKW basal medium, supplemented with 2.5 µM meta-topolin, grown under mixed LED light. The highest number of roots per plantlets was recorded on the rooting medium with 0.3 mgl-1 NAA.


This research is a part of bilateral Bulgaria-China project DHTC/01/2/2016, supported by National Science Fund, Ministry of Education and Science, Bulgaria.


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