Acta Univ. Agric. Silvic. Mendelianae Brun. 2020, 68, 119-127

https://doi.org/10.11118/actaun202068010119
Published online 2020-02-27

The Utilization of Vegetable and Fruit Wastes for Saccharomyces cerevisieae Cell Wall Based β-Glucan Production with Antioxidant Activity

Gemilang Lara Utama1, Florensia Irena1, Elazmanawati Lembong1, Indira Lanti Kayaputri1, Tensiska Tensiska1, Roostita Lobo Balia2

1Faculty of Agro-Industrial Technology, Universitas Padjadjaran, Jl. Raya Bandung Sumedang Km.21, 40600, Sumedang, Indonesia
2Faculty Animal Husbandry, Universitas Padjadjaran, Jl. Raya Bandung Sumedang Km.21, 40600, Sumedang, Indonesia

Received October 26, 2019
Accepted January 7, 2020

The study aims to determine the number of mass and antioxidant activity of β-glucan extracted from S.cerevisieae which grown on vegetable and fruit wastes. The method used is experimental with descriptive analysis which consisted of 3 treatments namely banana waste, papaya waste, and napa cabbage waste as fermentation medium, repeated thrice. Fermentation medium was made by mixing waste with water with the ratio of 1:2. Ten percent (w/v) of S.cerevisieae was inoculated and incubated for 48 hours, at 27 ℃. Extraction of β-glucan was carried out using acid-alkaline methods and antioxidant activity was tested by DPPH (2,2-Diphenyl-1-picrylhydrayl) method and the microstructure of β-glucan is determined by Scanning Electrone Microscope. The result showed that the best medium in producing β-glucan was papaya waste which resulting 19.094 g β-glucan mass, with radical scavenging activity of 20.71 % and globular diameter of 533 μm.

Funding

Author would like to thank the Student Research Group, Vivi Fadila Sari, Isfari Dinika and Syarah Virgina who helped in the laboratory. The research funded by the Directorate of Research and Community Service, Directorate General of Research Strengthening and Development, Ministry of Research, Technology and Higher Education the Republic of Indonesia, through the scheme “Penelitian Dasar” and “Academic Leadership Grant” of Universitas Padjadjaran.

References

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