Acta Univ. Agric. Silvic. Mendelianae Brun. 2019, 67, 1485-1491
Published online 2019-12-22

Routine Determination of Milk Fat Composition for Nutritional and Technological Purposes

Eva Samková1, Oto Hanuš2, Jiří Špička1, Lenka Pecová1, Jan Bedrníček1, Pavel Kopunecz3, Zdeňka Klímová3, Jaroslav Kopecký2

1University of South Bohemia in České Budějovice, Faculty of Agricultural, Branišovská 1645/31a, 370 05 České Budějovice, Czech Republic
2Dairy Research Institute, Ltd., Prague, Ke Dvoru 12a, 160 00 Praha 6-Vokovice, Czech Republic
3Czech Moravia Breeders Corporation, Benešovská 123, 252 09 Hradištko, Czech Republic

Received September 26, 2019
Accepted November 12, 2019

78 individual milk samples (IS) were taken in the Czech Fleckvieh and Holstein dairy cow herds and 33 bulk milk samples (BS) were taken in 13 Holstein dairy cow herds to compare two methods for the determination of fatty acids (FAs) – routine/indirect (infrared (IR) spectroscopy, MIR-FT) and reference/direct (gas chromatography, GC). The IS results show that the fatty acids C16:0 and C18:1 and the group of saturated (SFA) and unsaturated (UFA) FAs can be determined using routine method with a relatively high reliability (correlations (r) 0.7543, 0.7607, 0.8424 and 0.9492; P < 0.001). In case of polyunsaturated FAs is the r lower (0.2891; P < 0.05). In BS the tightness of relations is stronger in the case of the SFA and UFA (r 0.7094; 0.9389; P < 0.001), while for the group of TFA (trans isomers of unsaturated FAs) and PUFA (polyunsaturated FAs) are lower r (0.5897 and 0.5931; P < 0.001 respectively). Routine method for fatty acids profile determination is efficient for selection of raw milk to guarantee the production of specific dairy products with increased content of healthful fatty acids.


This paper was supported by projects MZe QK1910174, RO1419 and GAJU 028/2019/Z. Further the authors thank Mr. director Ass. Prof. Dipl. Eng. Josef Kučera, Mr. Dipl. Eng. Jan Zlatníček and Mr. Zdeněk Motyčka from ČMSCH a.s. for their professional and technical cooperation.


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